Jamestown is a city in Stutsman County, North Dakota in the United States. It is the county seat of Stutsman County^GR6. The population was 15,527 at the 2000 census. It currently sits as the seventh largest city in North Dakota. Jamestown was founded in 1872.

The local paper is the Jamestown Sun. Jamestown Regional Airport serves the city. Jamestown is home to Jamestown College, a private, four-year, liberal arts institution on the city's north side. On the south side of the city sits the North Dakota State Hospital. This hospital treats patients with psychiatric disorders, as well as serving as the state's holding facility for dangerously mentally-ill inmates. On the east side of the city and almost adjacent to the site of historic Fort Seward is The Anne Carlson School (formerly known as the "Crippled Childrens' School"). A privately-funded residential school, it has long been one of the country's leading centers for treatment and education of severely handicapped children. In fact, Jamestown was the first city in America to require wheelchair cutouts in newly-constructed sidewalk curbs.