Ames is a city in Story County, Iowa, United States. As of the 2000 Census, the city population was 50,731. The city was named after 19th century U.S. congressman Oakes Ames of Massachusetts, who was influential in the building of the transcontinental railroad. Ames was founded near a location that was deemed favorable for a railroad crossing of the Skunk River. It is located roughly 30 miles (48 km) north of the state capital Des Moines. Two small rivers run through the town: the Skunk River and Squaw Creek.

Ames is home of Iowa State University of Science & Technology, a space grant college, at its founding, the state's (Morrill Act) land-grant university, formerly known as the Iowa State College of Agriculture and Mechanic Arts. Ames is the home of the closely allied U.S Department of Agriculture's National Animal Disease Center, and the main offices of the Iowa state Department of Transportation. State and Federal institutions are the largest employers in Ames.

Other area employers include a 3M manufacturing plant; Sauer-Danfoss, a hydraulics manufacturer; Barilla, a pasta manufacturer; Pella a window manufacturer; and Ball, a manufacturer of canning jars and plastic bottles.